Development of genic and genomic microsatellites in Gleditsia triacanthos L. (Fabaceae) using Illumina sequencing

Authors

  • Yawen Wu Michigan Technological University, School of Forest Resources and Environmental Science, 1400 Townsend Drive, Houghton, MI 49931
  • Ruhua Zhang Michigan Technological University, School of Forest Resources and Environmental Science, 1400 Townsend Drive, Houghton, MI 49931, USA
  • Margaret Staton Department of Entomology and Plant Pathology, The University of Tennessee, Knoxville, TN 37996, USA
  • Scott E. Schlarbaum Department of Forestry, Wildlife and Fisheries, The University of Tennessee, Knoxville, TN 37996-4563, USA
  • Mark V. Coggeshall USDA Forest Service, Northern Research Station, Purdue University, West Lafayette, IN 47907, USA
  • Jeanne Romero-Severson Department of Biological Sciences, University of Notre Dame, Notre Dame, IN 46556, USA
  • John E. Carlson The Department of Ecosystem Science and Management and Department of Plant Science, Pennsylvania State University, University Park, PA 16802, SUA
  • Haiying Liang Department of Genetics and Biochemistry, Clemson University, Clemson, SC 29634, USA
  • Yi Xu Department of Plant Biology and Pathology, Rutgers University, New Brunswick, NJ 08901, USA
  • Daniela I. Drautz-Moses The Department of Genetics and Biochemistry, Clemson University, Clemson, SC 29634, USA
  • Stephan C. Schuster The Department of Genetics and Biochemistry, Clemson University, Clemson, SC 29634, USA
  • Oliver Gailing University of Göttingen, Forest Genetics and Forest Tree Breeding, Büsgenweg 2, 37077 Göttingen, Germany

DOI:

https://doi.org/10.15287/afr.2017.819

Keywords:

EST-SSRs, transcriptome, Gleditsia triacanthos, next-generation sequencing

Abstract

Twenty new polymorphic genic SSRs (EST-SSRs) and 13 genomic SSRs were developed in honeylocust (Gleditsia triacanthos) using Illumina transcriptome and low-coverage genome sequencing. A diversity panel of 40 honeylocust samples covering large parts of the species distribution range was characterized. As expected the level of genetic variation was lower in EST-SSRs than for non-genic genomic SSRs. This is the first report of EST-SSRs for honeylocust. All markers are polymorphic and produce clear single locus amplification products and can be used for genetic diversity and gene flow analyses. The transcriptome sequencing data provide a rich resource for new marker development.

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Published

2017-06-29

Issue

Vol. 60 No. 2 (2017)

Section

Research note

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