Influence of antibiotics on indirect organogenesis of Teak
DOI:
https://doi.org/10.15287/afr.2015.345Keywords:
Tectona grandis, co-cultivation, plant tissue culture, woody plantAbstract
Agrobacterium is the largest method employed to transform woody plants. The bacterium is required to introduce the transgene into the plant nuclear genome. After transferring T-DNA to the plant cell, the bacteria affect plant growth negatively and have to be eliminated from plant tissue culture medium through the use of antibiotics. The effect of different antibiotics (timentin, cefotaxime and carbenicillin) on in vitro shoot regeneration of teak (Tectona grandis L. f.) was compared in hypocotyl, mature cotyledon and cotyledonary segments explants. Timentin and cefotaxime (100-300 mg l-1) did not affect shoot regeneration and the number of shoots per explant. Moreover, at these concentrations, the two antibiotics seem to stimulate shoot regeneration. Carbenicillin at a dosage of 300 mg l-1 as well as cefotaxime and timentin at a dosage of 500 mg l-1 induced abundant calli formation and inhibited regeneration. Our data show that cefotaxime and timentin (300 mg l-1) can be harmless to teak regeneration and can be used as bactericide agents during Agrobacterium-mediated transformation of Tectona grandis. Furthermore, we discuss the effect of antibiotic degradation on plant morphogenesis and its effect on regeneration from different explants.References
Ascough G.D., Novák O., Pěnčík A., Rolčík J., Strnad M., Erwin J.E., Van Staden, J., 2009. Hormonal and cell division analyses in Watsonia lepida seedlings. J. Plant Physiol. 166:1497-1507. DOI: 10.1016/ j.jplph.2009.03.009 Baghel R.S., Tiwari S., Tripathi M.K., 2008. Comparison of morphogenic and plant regeneration ability of some explants of teak (Tectona grandis Linn. F). J. Agric. Tech. 4:125-136. Bosela M.J., 2009. Effects of β-lactam antibiotics, auxins, and cytokinins on shoot regeneration from callus cultures of two hybrid aspens, Populus tremuloides x P. tremula and P. x canescens x P. gradidentata. Plant Cell Tiss. Organ Cult. 98:249-261. DOI: 10.1007/ s11240-009-9555-5 Costa M.G.C., Nogueira F.T.S., Figueira M.L., Otoni W.C., Brommonschenkel S.H., Cecon P.R., 2000. Influence of the antibiotic Timentin on plant regeneration of tomato (Lycopersicon esculentum Mill.) cultivars. Plant Cell Rep. 19:327–332. DOI: 10.1007/ s002990050021 Fofana I.J., Ofori D., Poitel M., Verhaegen D., 2009. Diversity and genetic structure of teak (Tectona grandis L.f) in its natural range using DNA microsatellite markers. New For. 37:175–195. DOI: 10.1007/ s11056-008-9116-5 Giri C.C., Shyamkumar B., Anjaneyulu B. 2004. Progress in tissue culture, genetic transformation and applications of biotechnology to trees: an overview. Trees 18:115-135. DOI: 10.1007/s00468-003-0287-6 Gyves E.M., Royani J.I., Rugini E., 2007. Efficient method of micropropagation and in vitro rooting of teak (Tectona grandis L.) focusing on large-scale industrial plantations. Ann. For. Sci. 64:73–78. DOI: 10.1051/forest:2006090 Katayama N., Takano H., Sugiyama M., Takio S., SakaiA., Tanaka K., Kuroiwa H., Ono K. 2003. Effects of Antibiotics that Inhibit the Bacterial Peptidoglycan Synthesis Pathway on Moss Chloroplast Division. Plant Cell Physiol. 44:776-781. DOI: 10.1093/pcp/pcg096 Kong L., Dai D., Shang Li M.K., Zhang C.X., 2009. Thidiazuron-induced somatic embryos, their multiplication, maturation, and conversion in Cinnamomum pauciflorum Nees (Lauraceae). New For. 38:131–142. DOI: 10.1007/s11056-009-9135-x Kucharska D., Orlikowska T., 2009. Enhancement of in vitro organogenetic capacity of rose by preculture of donor shoots on the medium with thidiazuron. Acta Physiol. Plant 31:495–500. DOI: 10.1007/s11738-008-0258-z Le V.Q., Belles-Isles J., Dusabenyagasani M., Tremblay F.M., 2001. An improved procedure for production of white spruce (Picea glauca) transgenic plants using Agrobacterium tumefaciens. J. Exp. Bot. 52:2089-2095. Lin J-J., Assad-Garcia N., Kuo J., 1995. Plant hormone effect of antibiotics on the plant transformation efficiency of plant tissues by Agrobacterium tumefaciens cells. Plant Sci. 109:171–177. DOI: 10.1016/0168-9452(95)04168-T Ling H.Q., Kriseleit D., Ganal M.W., 1998. Effect of ticarcillin/potassium clavulanate on callus growth and shoot regeneration in Agrobacterium-mediated transformation of tomato (Lycopersicon esculentum Mill.). Plant Cell Rep. 17:843–847. DOI: 10.1007/s002990050495 Mamidala P., Nanna R.S., 2009. Efficient in vitro plant regeneration, flowering and fruiting of dwarf Tomato cv. Micro-Msk. Plant Omics J. 2:98-102. Mendes A.F.S., Cidade L.C., de Oliveira M.L.P., Otoni W.C., Soares-Filho W.S., Costa M.G.C., 2009. Evaluation of novel beta-lactam antibiotics in comparison to cefotaxime on plant regeneration of Citrus sinensis L. Osb. Plant Cell Tiss. Organ Cult. 97:331–336 DOI: 10.1007/s11240-009-9518-x Miranda J., Konschuh M.N., Yeung E.C., Chinnappa C.C., 1999. Invitro plantlet regeneration from hypocotyls explants of Stellaria longipes (Caryophyllaceae). Can.J. Bot. 77: 318–322. DOI: 10.1139/ cjb-77-2-318 Murashige T., Skoog F., 1962. Arevised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant 15: 473–497. DOI: 10.1111/j.1399-3054.1962.tb08052.x Murthy B.N.S., Murch S.J., Saxena P.K., 1998. Thidiazuron: A potent regulator of in vitro plant morphogenesis. In Vitro Cell. Dev. Biol. 34: 267-275. DOI: 10.1007/BF02822732 Nauerbey B., Billing K., Wyndaele R., 1997. Influence of the antibiotic Timentin on plant regeneration compared to carbenicillin and cefotaxime in concentrations suitable for elimination of Agrobacterium tumefaciens. Plant Sci. 123:169-177. DOI: 10.1016/S0168-9452(96)04569-4 Paiva Neto V.B., Mota T.R., Otoni W.C., 2003. Direct organogenesis from hypocotyl-derived explants of annatto (Bixa orellana). Plant Cell Tiss. Organ Cult. 75:159–167. DOI: 10.1023/A:1025063906822 Pollock K., Barfield D.G., Shields R., 1983. The toxity of antibiotics to plant cell cultures. Plant cell Rep. 2:36-39. Poupin M.J., Arce-Johnson P., 2005. Transgenic Trees for a New Era. In Vitro Cell. Dev. Biol.-Plant 41:91–101. DOI: 10.1079/IVP2004587 Recht M.I., Douthwaite S., Puglisi J.D., 1999. Basis for prokaryotic specificity of action of aminoglycoside antibiotics. The EMBO Journal 18:3133-3138. DOI: 10.1093/emboj/18.11.3133 Shirin F., Rana P.K., Mandal A.K., 2005. Invitro clonal propagation of mature Tectona grandis through axillary bud proliferation. J. For. Res. 10:465–469. DOI: 10.1007/s10310-005-0173-8 Souza B.M., Kraus J.E., Endres L., Mercier H., 2003. Relationships between endogenous hormonal levels and axillary bud development of Ananas comosus nodal segments. Plant Physiol. Bioch. 41:733–739. DOI: 10.1016/S0981-9428(03)00112-8 Tereso S., Miguel C., Maroco J., Oliveira M.M., 2006. Susceptibility of embryogenic and organogenic tissues of maritime pine (Pinus pinaster) to antibiotics used in Agrobacterium-mediated genetic transformation. Plant Cell Tiss. Organ Cult. 87:33-40. DOI: 10.1007/s11240-006-9130-2 Tiwari S.K., Tiwari K.P., Siril E.A., 2002. An improved micropropagation protocol for teak. Plant Cell, Tiss. Organ Cult. 71:1-6. DOI: 10.1023/A:1016570000846 Wightman F., Lighty D.L., 1982. Identification of phenylacetic acid as a natural auxin in the shoots of higher plants. Physiol. Plant. 55:17–24. DOI: 10.1111/j.1399-3054.1982.tb00278.x Zhi-neng L., Guo-feng L., Fang F., Man-zhu B., 2007. Adventitious shoot regeneration of Platanus acerifolia Willd. facilitated by Timentin, an antibiotic for suppression of Agrobacterium tumefaciens in genetic transformation. For. Stud. China9:14–18. DOI: 10.1007/s11632-007-0003-5
Published
Issue
Section
License
All the papers published in Annals of Forest Research are available under an open access policy (Gratis Gold Open Access Licence), which guaranty the free (of taxes) and unlimited access, for anyone, to entire content of the all published articles. The users are free to “read, copy, distribute, print, search or refers to the full text of these articles”, as long they mention the source.
The other materials (texts, images, graphical elements presented on the Website) are protected by copyright.
The journal exerts a permanent quality check, based on an established protocol for publishing the manuscripts. The potential article to be published are evaluated (peer-review) by members of the Editorial Board or other collaborators with competences on the paper topics. The publishing of manuscript is free of charge, all the costs being supported by Forest Research and Management Institute.
More details about Open Access:
Wikipedia: http://en.wikipedia.org/wiki/Open_access
