Research article

Climate as possible reproductive barrier in Pinus radiata (D. Don) interspecific hybridisation

Hannél Ham , Ben du Toit, Anna-Maria Botha, Arnulf Kanzler

Hannél Ham
Department of Forest and Wood Science, Stellenbosch University, Private Bag X1, Stellenbosch, 7602, South Africa. Email: hamh@sun.ac.za
Ben du Toit
Department of Forest and Wood Science, Stellenbosch University, Private Bag X1, Stellenbosch, 7602, South Africa
Anna-Maria Botha
Department of Forest and Wood Science, Stellenbosch University, Private Bag X1, Stellenbosch, 7602, South Africa & Genetics Department, Stellenbosch University, Private Bag X1, Stellenbosch, 7602, South Africa
Arnulf Kanzler
Department of Forest and Wood Science, Stellenbosch University, Private Bag X1, Stellenbosch, 7602, South Africa & Sappi Forest Research, P.O. Box 473, Howick 3290, South Africa

Online First: May 25, 2017
Ham, H., du Toit, B., Botha, A., Kanzler, A. 2017. Climate as possible reproductive barrier in Pinus radiata (D. Don) interspecific hybridisation. Annals of Forest Research DOI:10.15287/afr.2016.801


Historically, interspecific hybridisation with Pinus radiata D. Don had limited success. The effect of environmental conditions and position of pollination bags in the tree were investigated as possible hybridisation barriers. The study was conducted in a P. radiata seed orchard in the Southern Cape (South Africa). Field data were compared to the climatic conditions at natural and commercial provenances of seven Mesoamerican Pinus species identified as possible hybrid partners. In vitro pollen studies were used to confirm whether interspecific crosses with P. radiata might be feasible within predefined climatic parameters. The temperature ranges for both top and northern side of P. radiata pine trees in the seed orchard was similar to the natural distribution of P. radiata, P. elliottii Engelm. and P. taeda L. in the USA. Results suggested that pollen of P. elliottii and P. taeda might be more suited to result in the successful pollination of P. radiata than the other Mesoamerican pine species tested in this study.  Furthermore, the combination of minimum temperature and precipitation also showed a closer correlation to successful hybridisation with P. radiata for both P. elliotii and P. taeda. However, pollen tube elongation studies did not support these results, suggesting that mean temperature might not be the only determining factor of hybridisation success. Three circadian temperature models that mimic natural conditions were developed for Karatara and Sabie (Tweefontein, Witklip and Spitskop).  These models will be tested in future in vitro studies to further evaluate temperature fluctuations between day and night regimes as a possible reproductive barrier limiting hybridisation success between P. radiata and other Mesoamerican pine species.

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  • Hannél Ham
  • Ben du Toit
  • Anna-Maria Botha
  • Arnulf Kanzler
  • Hannél Ham
  • Ben du Toit
  • Anna-Maria Botha
  • Arnulf Kanzler