Research article

Adaptive genetic variability and differentiation of Croatian and Austrian Quercus robur L. populations at a drought prone field trial

Saša Bogdan , Mladen Ivanković, Martina Temunović, Maja Morić, Jozo Franjić, Ida Katičić Bogdan

Saša Bogdan
University of Zagreb, Faculty of Forestry, Department of Forest Genetics, Dendrology and Botany, Svetosimunska cesta 25, 10000 Zagreb, Croatia. Email: sbogdan@sumfak.hr
Mladen Ivanković
Croatian Forest Research Institute; Cvjetno naselje 41, 10450, Jastrebarsko, Croatia
Martina Temunović
University of Zagreb, Faculty of Forestry, Department of Forest Genetics, Dendrology and Botany, Svetosimunska cesta 25, 10000 Zagreb, Croatia
Maja Morić
Croatian Forest Research Institute; Cvjetno naselje 41, 10450, Jastrebarsko, Croatia
Jozo Franjić
University of Zagreb, Faculty of Forestry, Department of Forest Genetics, Dendrology and Botany, Svetosimunska cesta 25, 10000 Zagreb, Croatia
Ida Katičić Bogdan
University of Zagreb, Faculty of Forestry, Department of Forest Genetics, Dendrology and Botany, Svetosimunska cesta 25, 10000 Zagreb, Croatia

Online First: February 08, 2017
Bogdan, S., Ivanković, M., Temunović, M., Morić, M., Franjić, J., Katičić Bogdan, I. 2017. Adaptive genetic variability and differentiation of Croatian and Austrian Quercus robur L. populations at a drought prone field trial. Annals of Forest Research DOI:10.15287/afr.2016.733


Provenance trials, where populations of different geographical origin are tested in a common environment (common garden test), are a tool suited to allow the study of intraspecific adaptive genetic variation. Research of pedunculate oak (Quercus robur L.) adaptive genetic variability through analyses of populations in common garden tests has a long tradition. However, pedunculated oak populations originating south-eastern from the Alps have been scarcely studied in this way. This study addresses the adaptive genetic variability and differentiation of pedunculate oak populations originating from Austria and Croatia in a provenance/progeny field trial. Studied plants were six years old and were growing at the trial for three years. After two years of unusually low precipitations height and survival were analysed. The total mean height of all plants in the trial was 137.8 cm and ranged from 123.0 cm to 151.8 cm. The overall mean survival rate was rather high (0.85). Mean population survival ranged from 0.64 to 0.94. Individual narrow-sense heritabilities (hi2), family mean heritabilities (hf2), the coefficients of additive genetic variation (CVA) and quantitative genetic differentiation coefficients (QST) were calculated. A multivariate regression tree (MRT) analysis was used to determine the pattern of genetic differentiation of the populations. Individual heritabilities for height ranged between 0.00 and 0.39. Family mean heritabilities for height were rather low in most populations as well (<0.5). Family mean heritabilities for survival were higher than for height (ranging between 0.00 and 0.77). Calculated QST coefficients (0.25 for height and 0.14 for survival) indicated between-population genetic differentiation. The populations were separated into two clusters by MRT analysis regarding a climatic variable, namely Hargreaves’ reference evapotranspiration. Populations originating from comparatively more humid habitats were grouped in the first cluster. The first cluster had a lower mean height and survival compared to the second one. The differences between these clusters were highly statistically significant. The observed quantitative genetic differentiation might have been driven by natural selection caused by differences in the relative moisture of the habitats from which the progeny populations originate. The results suggest ecotypic pattern of the quantitative genetic differentiation among studied populations.


Arend M., Kuster T., Günthardt-Goerg M.S., Dobbertin M., 2011. Provenance-specific growth responses to drought and air warming in three European oak species (Quercus robur, Q. petraea and Q. pubescens). Tree Physiology 31:287–297. DOI: 10.1093/treephys/tpr004 Baliuckas V., Lagerström T., Eriksson G., 2001. Within-population variation in juvenile growth rhythm and growth in Quercus robur L. and Fagus sylvatica L. International Journal of Forest Genetics 8(4): 259-269. Baliuckas V, Pliura A., 2003. Genetic variation and phenotypic plasticity of Quercus robur populations and open-pollinated families in Lithuania. Scandinavian Journal of Forest Research 18: 305–319. DOI: 10.1080/02827580310005153 Baliuckas V, Pliūra A., 2008. Phenogenetic variation pattern in adaptive traits of Betula pendula, Alnus glutinosa and Quercus robur in Lithuania. Biologija 54: 60–65. DOI: 10.2478/v10054-008-0012-x Bartha D., 2010. The past, present and future tasks of Hungarian dendrological research. Acta Biologica Hungarica 61: 2–19. DOI: 10.1556/ABiol.61.2010.Suppl.2 Barzdajn W., 1993. Preliminary results of an experiment with Polish provenances of pedunculate oak (Quercus robur L.) and sessile oak (Q. petraea [Matt] Liebl.). Annales des Sciences Forestières 50(supplement): 222s-227s. DOI: 10.1051/forest:19930721 Barzdajn W., 2008. Comparison of provenance, family and individual heritability of growth traits in pedunculate oak (Quercus robur L.) in the family-provenance trial in the Milicz Forest District. Sylwan 5: 52–59. Bogdan S, Katicic-Trupcevic I, Kajba D., 2004. Genetic variation in growth traits in a Quercus robur L. open-pollinated progeny test of the Slavonian Provenance. Silvae Genetica 53: 198-201. De’Ath G., 2002. Multivariate regression trees: a new technique for modeling species-environment relationships. Ecology 83: 1105-1117. DHMZ., 2013. Izvješće Hrvatskog Hidrometeorološkog zavoda [Report of the Croatian Hydrometeorological Service]. Ocjena godine. http://klima.hr/ocjene_arhiva.php. (In Croatian). Ducousso A., Bordacs S., 2003. EUFORGEN Technical Guidelines for genetic conservation and use for pedunculate and sessile oaks (Quercus robur and Q. petraea). 6 p. Gailing O., Wachter H., Schmitt H., et al., 2007. Characterization of different provenances of Slavonian pedunculate oaks (Quercus robur L.) in Munsterland (Germany) with chloroplast DNA markers: PCR-RFLPs and chloroplast microsatellites. Allgemeine Forst und Jagdzeitung 178(5/6): 85-90. Hamann A., Gylander T., Chen P., 2011. Developing seed zones and transfer guidelines with multivariate regression trees. Tree Genetics & Genomes 7:399–408. DOI: 10.1007/s11295-010-0341-7 Hampe A., Petit R.J., 2005. Conserving biodiversity under climate change: the rear edge matters. Ecology Letters 8:461–467. DOI: 10.1111/j.1461-0248.2005.00739.x Van Hees A., 1997. Growth and morphology of pedunculate oak (Quercus robur L.) and beech (Fagus sylvatica L.) seedlings in relation to shading and drought. Annales des Sciences Forestières 54:9–18. DOI: 10.1051/forest:19970102 Houle D., 1992. Comparing evolvability and variability of quantitative traits. Genetics 130:195–204. Jensen J.S., 1993. Variation of growth in Danish provenance trials with oak (Quercus robur L. and Quercus petraea Mattuschka Liebl.). Annales des Sciences Forestières 50(supplement): 203-207. DOI: 10.1051/forest:19930718 Jensen J.S., 2000. Provenance variation in phenotypic traits in Quercus robur and Quercus petraea in Danish provenance trials. Scandinavian Journal of Forest Research 15:297–308. DOI: 10.1080/028275800447922 Jensen JS, Hansen JK., 2008. Geographical variation in phenology of Quercus petraea (Matt.) Liebl and Quercus robur L. oak grown in a greenhouse. Scandinavian Journal of Forest Research 23:179–188. DOI: 10.1080/02827580801995331 Jones A., Montanarella L., Jones R., 2005. Soil Atlas of Europe. European Commission. Luxembourg. 128 p. Katičić Bogdan, I., 2012. Genetska raznolikost hrasta lužnjaka (Quercus robur L.) u klonskim sjemenskim planatažama u Hrvatskoj [Genetic diversity of pedunculate oak (Quercus robur L.) in clonal seed orchards in Croatia]. Doctoral thesis. University of Zagreb, Faculty of Forestry. 166 p. (In Croatian with English abstract). Kleinschmit J., 1993. Intraspecific variation of growth and adaptive traits in European oak species. Annales des Sciences Forestières 50(Supplement): 166s-185s. DOI: 10.1051/forest:19930716 Koloszár J., 1987. Die slawonische Eiche in Ungarn [The Slavonic oak in Hungary]. Forst und Holzwirt 11: 293-296. Krahl-Urban J., 1959. The Oaks. A forestry monograph on Quercus robur and Q. sessitiflora, 288 pp. Kremer A., 2010. Evolutionary responses of European oaks to climate change. Irish Forestry 67:53–66. Lindner M., Maroschek M., Netherer S., Kremer A., Barbati A., Garcia-Gonzalo J., Seidl R., Delzon S., Corona P., Kolström M., Lexer M.J., 2010. Climate change impacts, adaptive capacity, and vulnerability of European forest ecosystems. Forest Ecology and Management, 259:698–709. DOI: 10.1016/j.foreco.2009.09.023 Matyas V., 1970. New oak taxa of Hungary. Acta Botanica Academiae Scientiarum Hungaricae 16:326–361. Maurer W.D., Tabel U., König A.O., Stephan B.R., Müller-Starck G., 2000. Provenance trials on Quercus robur L. and Quercus petraea (Matt.) Liebl. in Rhineland-Palatinate (Germany): preliminary results of phenotypic and genetic surveys. In: Vukelić J., Anić I. (eds.), Proceedings of the IUFRO Unit 2.08. 05 International conference’Oak 2000-Improvement of wood quality and genetic diversity of oaks’. Zagreb, Croatia, 20-25 May 2000, pp. 329–345. Parry M.L., 2007. Climate Change 2007: impacts, adaptation and vulnerability. Working Group II Contribution to the Fourth Assessment Report of the IPCC Intergovernmental Panel on Climate Change. Cambridge University Press. Popović M., Ivanković M., Bogdan S., 2014. Variability of height growth and survival of progeny from pedunculate oak (Quercus robur L.) seed stands at the field trial ‘Jastrebarski lugovi’ – first results. Šumarski list 1-2:155-165. Siegismund H.R., Jensen J.S., 2001. Intrapopulation and interpopulation genetic variation of Quercus in Denmark. Scandinavian Journal of Forest Research 16:103–116. DOI: 10.1080/028275801300088143 Spitze K., 1993. Population structure in Daphnia obtusa: quantitative genetic and allozymic variation. Genetics 135: 367–374. Thomas F.M., Gausling T., 2000. Morphological and physiological responses of oak seedlings (Quercus petraea and Q. robur ) to moderate drought. Annals of Forest Science 57:325–333. DOI: 10.1051/forest:2000123 Valkonen S., 2008. Survival and growth of planted and seeded oak (Quercus robur L.) seedlings with and without shelters on field afforestation sites in Finland. Forest Ecology and Management 255: 1085–1094. DOI: 10.1016/j.foreco.2007.10.038 Wang T., Hamann A., Spittlehouse D.L., Murdock T.Q., 2012. ClimateWNA-High-resolution spatial climate data for western North America. Journal of Applied Meteorology and Climatology 51:16–29. DOI: 10.1175/JAMC-D-11-043.1


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