Research note

Influence of antibiotics on indirect organogenesis of Teak

Evandro Vagner Tambarussi , Marcelo Rogalski, Fábio Tebaldi Silveira Nogueira, Gilvano Ebling Brondani, Valentina de Fátima De Martin, Helaine Carrer

Evandro Vagner Tambarussi
Universidade Federal de São Carlos, Departamento de Biologia, Rod. João Leme dos Santos, Km 110, Sorocaba, SP, 18052-780, Brazil. Email:
Marcelo Rogalski
Universidade Federal de Viçosa, Brazil
Fábio Tebaldi Silveira Nogueira
Universidade de São Paulo, Brazil
Gilvano Ebling Brondani
Universidade Federal do Mato Grosso, Brazil
Valentina de Fátima De Martin
Universidade de São Paulo, Brazil
Helaine Carrer
Universidade de São Paulo, Brazil

Online First: March 09, 2015
Tambarussi, E., Rogalski, M., Nogueira, F., Brondani, G., De Martin, V., Carrer, H. 2015. Influence of antibiotics on indirect organogenesis of Teak. Annals of Forest Research DOI:10.15287/afr.2015.345

Agrobacterium is the largest method employed to transform woody plants. The bacterium is required to introduce the transgene into the plant nuclear genome. After transferring T-DNA to the plant cell, the bacteria affect plant growth negatively and have to be eliminated from plant tissue culture medium through the use of antibiotics. The effect of different antibiotics (timentin, cefotaxime and carbenicillin) on in vitro shoot regeneration of teak (Tectona grandis L. f.) was compared in hypocotyl, mature cotyledon and cotyledonary segments explants. Timentin and cefotaxime (100-300 mg l-1) did not affect shoot regeneration and the number of shoots per explant. Moreover, at these concentrations, the two antibiotics seem to stimulate shoot regeneration. Carbenicillin at a dosage of 300 mg l-1 as well as cefotaxime and timentin at a dosage of 500 mg l-1 induced abundant calli formation and inhibited regeneration. Our data show that cefotaxime and timentin (300 mg l-1) can be harmless to teak regeneration and can be used as bactericide agents during Agrobacterium-mediated transformation of Tectona grandis. Furthermore, we discuss the effect of antibiotic degradation on plant morphogenesis and its effect on regeneration from different explants.

Ascough G.D., Novák O., Pěnčík A., Rolčík J., Strnad M., Erwin J.E., Van Staden, J., 2009. Hormonal and cell division analyses in Watsonia lepida seedlings. J. Plant Physiol. 166:1497-1507. DOI: 10.1016/ j.jplph.2009.03.009

Baghel R.S., Tiwari S., Tripathi M.K., 2008. Comparison of morphogenic and plant regeneration ability of some explants of teak (Tectona grandis Linn. F). J. Agric. Tech. 4:125-136.

Bosela M.J., 2009. Effects of β-lactam antibiotics, auxins, and cytokinins on shoot regeneration from callus cultures of two hybrid aspens, Populus tremuloides x P. tremula and P. x canescens x P. gradidentata. Plant Cell Tiss. Organ Cult. 98:249-261. DOI: 10.1007/ s11240-009-9555-5

Costa M.G.C., Nogueira F.T.S., Figueira M.L., Otoni W.C., Brommonschenkel S.H., Cecon P.R., 2000. Influence of the antibiotic Timentin on plant regeneration of tomato (Lycopersicon esculentum Mill.) cultivars. Plant Cell Rep. 19:327–332. DOI: 10.1007/ s002990050021

Fofana I.J., Ofori D., Poitel M., Verhaegen D., 2009. Diversity and genetic structure of teak (Tectona grandis L.f) in its natural range using DNA microsatellite markers. New For. 37:175–195. DOI: 10.1007/ s11056-008-9116-5

Giri C.C., Shyamkumar B., Anjaneyulu B. 2004. Progress in tissue culture, genetic transformation and applications of biotechnology to trees: an overview. Trees 18:115-135. DOI: 10.1007/s00468-003-0287-6

Gyves E.M., Royani J.I., Rugini E., 2007. Efficient method of micropropagation and in vitro rooting of teak (Tectona grandis L.) focusing on large-scale industrial plantations. Ann. For. Sci. 64:73–78. DOI: 10.1051/forest:2006090

Katayama N., Takano H., Sugiyama M., Takio S., SakaiA., Tanaka K., Kuroiwa H., Ono K. 2003. Effects of Antibiotics that Inhibit the Bacterial Peptidoglycan Synthesis Pathway on Moss Chloroplast Division. Plant Cell Physiol. 44:776-781. DOI: 10.1093/pcp/pcg096

Kong L., Dai D., Shang Li M.K., Zhang C.X., 2009. Thidiazuron-induced somatic embryos, their multiplication, maturation, and conversion in Cinnamomum pauciflorum Nees (Lauraceae). New For. 38:131–142.
DOI: 10.1007/s11056-009-9135-x

Kucharska D., Orlikowska T., 2009. Enhancement of in vitro organogenetic capacity of rose by preculture of donor shoots on the medium with thidiazuron. Acta Physiol. Plant 31:495–500. DOI: 10.1007/s11738-008-0258-z

Le V.Q., Belles-Isles J., Dusabenyagasani M., Tremblay F.M., 2001. An improved procedure for production of white spruce (Picea glauca) transgenic plants using Agrobacterium tumefaciens. J. Exp. Bot. 52:2089-2095.

Lin J-J., Assad-Garcia N., Kuo J., 1995. Plant hormone effect of antibiotics on the plant transformation efficiency of plant tissues by Agrobacterium tumefaciens cells. Plant Sci. 109:171–177. DOI: 10.1016/0168-9452(95)04168-T

Ling H.Q., Kriseleit D., Ganal M.W., 1998. Effect of ticarcillin/potassium clavulanate on callus growth and shoot regeneration in Agrobacterium-mediated transformation of tomato (Lycopersicon esculentum Mill.). Plant Cell Rep. 17:843–847. DOI: 10.1007/s002990050495

Mamidala P., Nanna R.S., 2009. Efficient in vitro plant regeneration, flowering and fruiting of dwarf Tomato cv. Micro-Msk. Plant Omics J. 2:98-102.

Mendes A.F.S., Cidade L.C., de Oliveira M.L.P., Otoni W.C., Soares-Filho W.S., Costa M.G.C., 2009. Evaluation of novel beta-lactam antibiotics in comparison to cefotaxime on plant regeneration of Citrus sinensis L. Osb. Plant Cell Tiss. Organ Cult. 97:331–336 DOI: 10.1007/s11240-009-9518-x

Miranda J., Konschuh M.N., Yeung E.C., Chinnappa C.C., 1999. Invitro plantlet regeneration from hypocotyls explants of Stellaria longipes (Caryophyllaceae). Can.J. Bot. 77: 318–322. DOI: 10.1139/ cjb-77-2-318

Murashige T., Skoog F., 1962. Arevised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant 15: 473–497. DOI: 10.1111/j.1399-3054.1962.tb08052.x

Murthy B.N.S., Murch S.J., Saxena P.K., 1998. Thidiazuron: A potent regulator of in vitro plant morphogenesis. In Vitro Cell. Dev. Biol. 34: 267-275. DOI: 10.1007/BF02822732

Nauerbey B., Billing K., Wyndaele R., 1997. Influence of the antibiotic Timentin on plant regeneration compared to carbenicillin and cefotaxime in concentrations suitable for elimination of Agrobacterium tumefaciens. Plant Sci. 123:169-177. DOI: 10.1016/S0168-9452(96)04569-4

Paiva Neto V.B., Mota T.R., Otoni W.C., 2003. Direct organogenesis from hypocotyl-derived explants of annatto (Bixa orellana). Plant Cell Tiss. Organ Cult. 75:159–167. DOI: 10.1023/A:1025063906822

Pollock K., Barfield D.G., Shields R., 1983. The toxity of antibiotics to plant cell cultures. Plant cell Rep. 2:36-39.

Poupin M.J., Arce-Johnson P., 2005. Transgenic Trees for a New Era. In Vitro Cell. Dev. Biol.-Plant 41:91–101. DOI: 10.1079/IVP2004587

Recht M.I., Douthwaite S., Puglisi J.D., 1999. Basis for prokaryotic specificity of action of aminoglycoside antibiotics. The EMBO Journal 18:3133-3138. DOI: 10.1093/emboj/18.11.3133

Shirin F., Rana P.K., Mandal A.K., 2005. Invitro clonal propagation of mature Tectona grandis through axillary bud proliferation. J. For. Res. 10:465–469. DOI: 10.1007/s10310-005-0173-8

Souza B.M., Kraus J.E., Endres L., Mercier H., 2003. Relationships between endogenous hormonal levels and axillary bud development of Ananas comosus nodal segments. Plant Physiol. Bioch. 41:733–739. DOI: 10.1016/S0981-9428(03)00112-8

Tereso S., Miguel C., Maroco J., Oliveira M.M., 2006. Susceptibility of embryogenic and organogenic tissues of maritime pine (Pinus pinaster) to antibiotics used in Agrobacterium-mediated genetic transformation. Plant Cell Tiss. Organ Cult. 87:33-40. DOI: 10.1007/s11240-006-9130-2

Tiwari S.K., Tiwari K.P., Siril E.A., 2002. An improved micropropagation protocol for teak. Plant Cell, Tiss. Organ Cult. 71:1-6. DOI: 10.1023/A:1016570000846

Wightman F., Lighty D.L., 1982. Identification of phenylacetic acid as a natural auxin in the shoots of higher plants. Physiol. Plant. 55:17–24. DOI: 10.1111/j.1399-3054.1982.tb00278.x

Zhi-neng L., Guo-feng L., Fang F., Man-zhu B., 2007. Adventitious shoot regeneration of Platanus acerifolia Willd. facilitated by Timentin, an antibiotic for suppression of Agrobacterium tumefaciens in genetic transformation. For. Stud. China9:14–18. DOI: 10.1007/s11632-007-0003-5

No Supplimentary Material available for this article.
No metrics available for this article.